Figure 3.
The signaling induced by the LIBS-specific antibodies is not Fc receptor (FcγRIIA)–dependent. (A) Aggregation traces of normal and VGTΔ724 platelets treated with D3 (30 μg/mL) plus Fg (250 μg/mL) in the presence of protein G (15 μg/mL). (B) Aggregation traces of normal and VGTΔ724 platelets treated with D3 (30 μg/mL) plus donkey anti–mouse polyclonal antibody (Ab; 30 μg/mL) with or without protein G. (C) TxA2 production (top) and ATP secretion (bottom) by normal and VGTΔ724 platelets in response to different treatments which inhibit or enhance Fc receptor–mediated signaling. Data shown here were from 3 experiments. Error bars represent SD, n = 3. In some cases, the values of the SD were so small that the bars cannot be seen.