Table 2.

Effect of 8-Cl-Ado on RNA transcripts of apoptotic proteins

	% of control
MCL-1		BCL-2
Patient no.	4 h	24 h	4 h	24 h
15	72	49	ND	ND
16	62	59	92	114
17	81	64	107	49
18	143	118	108	152
19	113	129	80	118
20	89	98	98	105
21	4*	0	ND	ND
22	68*	0	ND	ND

Total RNA was isolated from CLL lymphocytes (1 × 10⁷/mL) after treatment with 10 μM 8-Cl-Ado at the indicated times. Real-time RT-PCR experiments were carried out to measure the RNA transcripts of antiapoptotic proteins MCL-1 and BCL-2. The values are expressed as percentage of control (untreated) cells.

ND indicates not done.

^*These values were measured at 18 hours in poly(A)-enriched RNA rather than total RNA