Figure 5.

Ectopic GATA-1 expression in human T_N,T_CM, and T_EM cells induces a Th2 cytokine profile. CD45RO^–RA⁺ naive and CD45RO⁺RA^– memory T cells were purified from adult blood PBMCs of healthy donors. Memory T cells were further sorted by FACS for expression of CCR7 into T_CM (CCR7⁺) and T_CM (CCR7^–) cells. The T-cell populations were TCR activated and transduced with HDV, HDV.GATA-1, or HDV.GATA-3 at the time of activation (see “Materials and methods”). Following 8 to 10 days of expansion, intracellular expression of the cytokines IFNγ, IL-4, IL-5, and IL-13 were determined by flow cytometry. For these experiments transduced cells were left unsorted, but mCD24⁺ cells were identified and gated on by costaining with an anti-mCD24 antibody. These data are representative of 3 separate experiments using T-cell subsets purified from different adult donors.