Table 3.
Colony-inhibition assays
| Effector | HLA match of E/T (n) | N | Target CD34+ | % colony inhibition | *P |
|---|---|---|---|---|---|
| CD56+ donor, CD56+ autologous | Matched | 11,3 | CML | 42 ± 9 | NS |
| CD56+ donor | Mismatched | 14 | CML | 39 ± 7 | NS |
| CD56+ donor | Matched (11); mismatched (4) | 15 | CML class I–negative, acid stripped | 69.8 ± 7 | .01 |
| CD56+ donor | Matched (11); mismatched (4) | 15 | CML class I–positive | 39.6 ± 10 | .01 |
| CD56+CD3- (NK) | Auto (1), matched (1) | 3 | CML | 69.1 ± 12 | NS |
| CD56+ CD3+ (NKT) | Mismatched (1) | 3 | CML | 68 ± 16 | NS |
| CD56+ donor, HLA-C group homozygous | Matched (4) | 4 | CML | 55.5 ± 10 | NS |
| CD56+ donor, HLA-C group heterozygous | Matched (4) | 4 | CML | 53 ± 7 | NS |
Colony inhibition calculated as mean CFU-GM plated after 4-hour CD56+ incubation / mean CFU-GM after 4 hours without CD56+ cells. The CML CD34+ cell dose was selected to obtain 40 to 100 colonies/plate (mean of duplicate plates).
CD56+ indicates NK+NK-T cells sorted from leukapheresis product or PB; E/T, effector-to-target.
*
Paired t test