Table 2.
Quantitative relative expression level of 4p16.3 genes from cell lines
| Cell line | TACC3 | FGFR3 | LETM1 | MMSET total | RE-IIBP | WHSC2 |
|---|---|---|---|---|---|---|
| Daudi | 1.51 | 0.00 | 1.02 | 1.02 | 1.44 | 1.41 |
| Raji | 1.00 | 0.00 | 1.00 | 1.00 | 1.00 | 1.00 |
| RPMI-8226 | 1.51 | 0.00 | 1.26 | 1.11 | 1.65 | 1.17 |
| U266 | 1.16 | 0.00 | 1.23 | 1.21 | 1.98 | 0.94 |
| KMS-12BM | 0.81 | 0.16 | 1.01 | 0.74 | 0.76 | 0.98 |
| KMS-12PE | 1.20 | 0.21 | 1.63 | 1.18 | 1.51 | 1.42 |
| KMS-11 [MB4-1, POS] | 1.01 | 87.86 | 0.36 | 2.13 | 4.27 | 0.51 |
| NCI-H929 [MB4-2, POS] | 3.61 | 4.23 | 1.04 | 2.16 | 4.85 | 1.03 |
| KMS-18 [MB4-2, POS] | 0.79 | 100.00 | 6.44 | 4.65 | 13.83 | 0.47 |
| JIM3 [MB4-2, NEG] | 0.33 | 0.02 | 1.12 | 7.87 | 14.45 | 0.39 |
| OPM-2 [MB4-3, POS] | 1.82 | 64.47 | 0.54 | 5.39 | 12.82 | 1.13 |
| LP-1 [MB4-2,* POS] | 1.52 | 0.80 | 5.19 | 1.47 | 2.32 | 1.81 |
The relative expression levels of all reactions, except FGFR3, are comparisons versus the expression level detected in Raji, which is set to an expression level of 1. For FGFR3, KMS-18, which had the highest level of expression, was used as the normalizer and was set at an expression level of 100. The panel of cell lines includes 2 Burkitt lymphoma lines (Daudi, Raji), 4 t(4;14)NEG MM cell lines (RPMI-8226, U266, KMS-12-BM, KMS-12-PE), and 6 t(4;14)POS MM cell lines (KMS-11, NCI-H929, KMS-18, JIM3, OPM-2, LP-1). For t(4;14)POS samples, the breakpoint type and FGFR3 expression status are indicated in brackets.
A unique der(4) breakpoint that occurs in a downstream switch region that did not rearrange with switch mu, which increases the distance between the mu enhancer and genes from chromosome 4