Table 1.
Identification by mass spectrometry of adaptors and structural proteins involved in cytoskeleton organization in NPM-ALK–positive cells
| NCBI accession no. | Identified protein | No. of peptide matches | Sequence coverage, % | IP | MW, kDa | MALDI identification | MS/MS identification |
|---|---|---|---|---|---|---|---|
| NP_0055382 | p130 Crk-associated substrate (p130Cas) | 3 | 6 | P-Tyr | 93 | – | + |
| AAH08633 | Actin, beta | 9 | 29 | P-Tyr | 41 | + | + |
| AAC16672 | Alpha-actinin-2-associated LIM protein | 6 | 21 | P-Tyr | 34 | + | – |
| AAA61279 | Vimentin | 15 | 32 | ALK | 53 | + | + |
| P05217 | Tubulin, beta | 9 | 28 | ALK | 48 | + | + |
| AAC51654 | Myosin VI | 8 | 8 | P-Tyr | 147 | + | + |
| NP_005955 | Myosin heavy chain, nonmuscle type B | 7 | 5 | ALK | 227 | + | + |
| NP_002464 | Myosin, heavy polypeptide 9, nonmuscle | 22 | 16 | ALK | 229 | + | – |
| NP_006650 | Tubulin, gamma complex-associated protein 2 | 2 | 2 | ALK | 103 | – | + |
| NP_004915 | Actinin alpha 4 | 2 | 7 | ALK | 104 | – | + |
| NP_055140 | Coronin 1C | 2 | 4 | ALK | 53 | – | + |
| NP_002009 | Flightless 1 homolog | 3 | 2 | ALK | 144 | – | + |
| NP_056065 | Coiled-coil protein BICD2 | 7 | 10 | P-Tyr | 93 | – | + |
| NP_006089 | RACK1 | 3 | 9 | P-Tyr | 35 | – | + |
IP indicates immunoprecipitation; MW, molecular weight.