Figure 1.
Analysis of T-cell repertoire. (A) CDR3 size distribution of TCRVβ subfamilies. Each TCRVβ fragment was amplified from cDNA with 1 of 24 Vβ-specific primers. The size distribution of polymerase chain reaction (PCR) products was determined by an automated sequencer and GeneScan software. (B) Complexity scores of TCRVβ. Complexity scores were generated for each TCRVβ from the CDR3 size distribution analysis.7 (C) Frequency of skewed TCRVβ repertoire. Shown are the mean (± standard deviation [SD]) numbers of skewed TCRVβ obtained from the control and patient groups. *P < .05.