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. 2005 Jun 9;106(6):1956–1964. doi: 10.1182/blood-2005-02-0657

Figure 2.

Figure 2.

Size distribution of HS. HSs were purified separately from each proteoglycan peak that was resolved by anion-exchange HPLC, and subjected to gel filtration chromatography on a CL-6B column, as described in “Materials and methods.” Three regions containing large-, intermediate-, and small-sized molecules were identified, based on the elution profile of normal intracellular HPLC peak no. 1 HS (Kav 0.1-0.4, 0.4-0.55, and 0.55-0.9, respectively [Kav = (elution volume - void volume)/(total volume - void volume)]). The proportion of HS present in each of these regions was calculated as a percentage of the total, for HS from intracellular peak no. 2 as well as HS from HPLC peaks no. 1 and no. 2 from the ECM.