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Mig does not inhibit agonist-induced calcium mobilization. Calcium transients in purified murine eosinophils were measured by labeling cells with Fura-2 AM, and monitoring immediately following stimulation with 50 nM eotaxin-1, 25 nM eotaxin-2 (A-B), and 10 nM PAF (C). Prestimulation with Mig (200 nM) has no effect on eotaxin- (B) or PAF-induced calcium mobilization (C). Data are representative of 3 experiments.
