Table 1.
Genes down-regulated in clo mutant embryos
| GenBank ID | Microarray ratio | RT-PCR ratio | Gene | Chromosome | Genomic region |
|---|---|---|---|---|---|
| BI704361 | 0 | 0.02 ± 0.01 | Zinc finger CW-type coiled-coil domain protein 3 (Zcw3)* | 20 | 14567660-14568523 |
| BI980779 | 0 | 0.24 ± 0.04 | Unknown | 14 | 11255408-11256040 |
| AW115759 | 0.04 | 0.01 | Novel, C1qR-like (crl), distantly similar to the complement C1q receptor precursor† | 17 | 6659458-6661171 |
| AF045432 | 0.04 | ND | T-cell acute lymphocytic leukemia 1 (tal1;scl)‡ | ND | ND |
| AI626636 | 0.07 | 0.04 ± 0.01 | Similar to adrenomedullin receptor (admr)† | 23 | 39810187-39812236 |
| U18311 | 0.08 | 0.01 ± 0.00 | GATA-binding protein 1 (gata1)‡ | ND | ND |
| AI793509 | 0.09 | ND | Novel ETS domain-containing protein (ETS1-related protein, etsrp)† | 16 | 33961499-33966546 |
| AF157109 | 0.09 | 0.23 | Draculin (drl)‡ | ND | ND |
| AI384298 | 0.13 | 0.17 ± 0.09 | Similar to scavenger receptor class F, member 1 (scarf1)*† | ND | ND |
| AI965222 | 0.16 | 0.04 ± 0.01 | Cadherin 5 (cdh5)† | 14 | 49119878-49123369 |
| AI105766 | 0.16 | 0.35 ± 0.07 | Similar to aquaporin 8† | 12 | 15316616-15376296 |
| AF191560 | 0.17 | 0.13 ± 0.02 | LIM domain only 2 (Imo2)‡ | ND | ND |
| AI353708 | 0.20 | ND | Unknown | ND | ND |
| AI959504 | 0.20 | 0.28 ± 0.01 | Similar to EGF, latrophilin and 7-TM domain protein 1 (etl1) | 11 | 6244483-6269257 |
| AJ011790 | 0.32 | 0.33 ± 0.08 | Claudin g | 24 | 13093172-13091270 |
| AW077654 | 0.32 | 0.01 | Spi1 (pu.1)‡ | ND | ND |
| BI896312 | 0.38 | 0.68 ± 0.03 | Friend leukemia integration 1 (fli1)‡ | ND | ND |
| AI353083 | 0.40 | ND | Hemoglobin alpha embryonic-3 (hbae3)‡ | ND | ND |
| BI842407 | 0.44 | 0.39 ± 0.02 | Unknown | 13 | 15277643-15277861 |
| AI601685 | 0.45 | 0.53 ± 0.03 | Similar to dual specificity phosphatase 5 (dusp5)† | 22 | 30622712-30633737 |
| BE693186 | 0.45 | ND | Hemoglobin alpha embryonic-1 (hbae1)‡ | ND | ND |
| AJ249590 | 0.48 | ND | Friend leukemia integration 1 (fli1)‡ | ND | ND |
| AF262978 | 0.49 | ND | Kruppel-like factor 4 (klf4; biklf)‡ | ND | ND |
| AF082662 | 0.49 | ND | Hemoglobin beta embryonic-1 (hbbe1)‡ | ND | ND |
| AJ236884 | 0.56 | 0.56 ± 0.09 | Retinol binding protein 4 (rbp4)† | ND | ND |
Ratios of gene expression in clo mutant embryos versus their siblings were calculated. Chromosomal location was determined using the zebrafish genome sequencing assembly Zv4 release.
ND indicates not determined.
*
Annotation of these noncoding 3′ UTR sequences is based on their genomic location in the immediate vicinity of the corresponding gene.
†
Genes selected for further characterization.
‡
Zebrafish genes previously known to be expressed in hematopoietic or vascular endothelial cells.