Table 3.

Summary of NOD-SCID engraftment

Sample no.*	No. engrafted/injected, unsorted	% human CD45, unsorted	Engrafted/injected, sorted	% human CD45, sorted	Mutant-wild-type ratio of FLT3
1	4/4	3/4	1.07
Mouse 1	90	22
Mouse 2	90	20
Mouse 3	90	4
Mouse 4	84
2	0/4	ND	ND	ND	N/A
3	4/4	4/4	All mutant
Mouse 1	97	95
Mouse 2	89	60
Mouse 3	80	52
Mouse 4	70	4
4	4/4	0/4	ND	0.81
Mouse 1	20
Mouse 2	10
Mouse 3	9
Mouse 4		5

Six AML samples were subjected to immunomagnetic selection for CD34⁺/CD38⁻ cells. The sample numbers shown are the same as in Table 1. For each sample, 4 sublethally irradiated NOD-SCID mice were injected with either 1 × 10⁶ unsorted cells or 5 × 10³ CD34⁺/CD38⁻ cells (thus, 12 groups, of 4 mice each). At 14 weeks, the mice were killed and the bone marrow cells were analyzed for expression of human CD45. Genomic DNA prepared from these marrows were analyzed for FLT3 mutant-wild-type ratio as described in “Materials and methods.” Engrafted/injected refers to the number of mice that engrafted, as defined by having detectable human CD45 staining, versus the number injected within that group.

ND indicates no engraftment detected.

^*For sample nos. 7 and 8, 0 of 4 mice were engrafted/injected with either sorted or unsorted cells, and thus results are not shown for these groups in the table.