Table 5.

CEP-701 has little effect on engraftment of normal hematopoietic stem cells

Normal donor CD34+ cells
Vehicle control, %CD45	CEP-701-treated, %CD45
57.1	13.6
34.5	15.6
69.9	58.0
9.5	14.2
44.2
43.0*	25.4*

CD34⁺ cells (6 × 10⁶) obtained from the mobilized peripheral blood of a healthy human donor were injected into 2 groups of 5 irradiated NOD-SCID mice. Mice were then treated with CEP-701 (20 mg/kg) or vehicle twice daily for 5 days out of each month. One mouse in the CEP-701 group died of apparent infection in the first month of the experiment (CEP-701, given subcutaneously, acts as a considerable skin irritant in these mice, which may have predisposed them to infection). At 14 weeks, mice were killed and the bone marrow cells were analyzed by flow cytometry for human CD45 staining. The % CD45 refers to the percentage of the total marrow that stained for human CD45 (as compared with murine CD45).

^*Indicates the mean value for the mice in that group.