Figure 1.
Engraftment of primary patient WM cells in SCID-hu mice. Blood was collected from mouse tail vein at different time points following inoculation of tumor cells directly in the human fetal bone chips, and sera were serially tested for circulating human IgM, IgG, and κ and λ chain by ELISA. The results demonstrate the appearance and increase in paraprotein, human IgM and/or light chain, in 3 representative SCID-hu mice, respectively, injected with patient IgMκ, IgMλ, and IgMκ WM cells. Panels A and B refer to mice injected with 5 and 10 × 106 BM cells, respectively, and panel C refers to a mouse injected with 2 × 106 CD19+-sorted cells.