Table 3.
Summary of RNAi experiments
| dsRNA injected | Wild type | sel-12(ar171)* | sel-12(ar131) |
|---|---|---|---|
| none† | + (many) | Egl (4/4) | Egl (5/5) |
| spr-2 | ND | Egl+ (6/15) | Egl+ (10/10) |
| D2096.8 | ND | Egl (13/13) | Egl (10/10) |
| spr-2 + D2096.8‡ | + (10/10) | Egl+ (9/15) | Egl+ (10/10) |
See text for details. +, no novel phenotypes, such as overt lethality, were seen. Egl, egg-laying defective. Egl+, egg-laying ability was restored, and no novel phenotypes were seen. The numbers in parentheses indicate the number of broods displaying the phenotype indicated/total injected hermaphrodites.
*
Also carried unc-1(e538).
†
Distilled water lacking dsRNA was injected.
‡
The extent of suppression of the Egl phenotype sel-12(ar171) and sel-12(ar131) was comparable to the extent of suppression seen with spr-2(RNAi) alone (data not shown).