Figure 4.

Multilineage blood differentiation 7 months after transplantation. (A) Peripheral blood chimerism, measured by FACS, is shown arising from 200 transplanted liver CD45⁺ SP tip cells (from a CD45.1 donor) over a 7-month period (n = 4 mice per group per experiment, repeated twice; error bars reflect standard deviation of the mean). (B) Representative FACS analysis of peripheral blood at 7 months shows robust engraftment of differentiated lymphoid (CD3⁺ or B220⁺), myeloid (CD11b⁺ or GR-1⁺), and erythroid (Ter-119⁺) hematopoietic lineages derived from the transplanted donor (CD45.1⁺) cells. Numbers in dot plots represent percentage of cells within each quadrant.