Figure 2.

MN1-TEL–expressing mice develop altered myelopoiesis de novo. (A) Increased GFP⁺ cells in BM of MN1-TEL–expressing mice, which died of severe anemia without T-lymphoid tumor (anemic cases). Numbers indicated are mean ± SE percentage of GFP⁺ cells (n = 3). MT/Cre indicates control MN1-TEL/Mx1-Cre BM cells 1 month (1 mo.) or 8 months (8 mo.) after treatment with pI-pC. (B) Increased Mac1⁺Gr1^- cells in MN1-TEL–expressing mice. The entire population of BM cells was analyzed by FCM. (Left) Age-matched control MN1-TEL/Mx1-Cre BM cells not induced with pI-pC. (Right) BM cells of MN1-TEL–expressing mice with increased number of GFP⁺ cells. Numbers indicated are mean ± SE percentage (n = 3). (C) Mac1⁺/Gr1^- cells in GFP⁺ or GFP^- BM cells of MN1-TEL–expressing mice with increased number of GFP⁺ cells. Gates were set to select the 10% GFP-brightest or -dimmest population in MN1-TEL–expressing mice to clearly discriminate MN1-TEL⁺ and MN1-TEL^- cells. Numbers indicated are mean ± SE percentage (n = 3). (Top) Mac1/Gr1 expression in GFP⁺ or GFP^- BM cells. (Bottom) FSC and SSC of Mac1⁺/Gr1^- cells in GFP⁺ or GFP^- BM cells.