Figure 4.

Characteristics of the myeloproliferative disease induced by coexpression of MN1-TEL and IL-3. (A) Myeloproliferation in mice that received transplants of MN1-TEL⁺/IL-3 cells. (Left) Hematoxylin-Eosin (H-E) staining of BM; (middle) myeloperoxidase staining of BM; (right) M-G staining of BM cells. An arrow indicates a cell with differentiated morphology. (B) Aggressive organ infiltration by myeloid cells in mice that received transplants of MN1-TEL⁺/IL-3 bone marrow. H-E staining of liver (left), spleen (middle), and lung (right). Arrows indicate infiltrations of the myeloid cells. Images were obtained as previously described.¹² (C) GFP and YFP expression in BM cells from mice that received transplants of MN1-TEL⁺/IL-3 cells. FCM analysis was performed as described in Figure 3A. (D) Clonality of MN1-TEL⁺/IL-3⁺ myeloproliferative disease (MPD). Genomic DNA of MPD BM cells was digested with BglII and hybridized with an Il3 probe. Control indicates untransduced BM; and G, germ line band.