Table 1.
Detection and quantification of HTLV-1 DNA in rabbit PBMCs
|
Inoculum and rabbit
|
Weeks after inoculation
|
||||
|---|---|---|---|---|---|
| 0 | 2 | 4 | 6 | 8* | |
| 729.HTLV-1 | |||||
| R1 | - | + (0.001) | + | + | + (0.112) |
| R2 | - | + (0.001) | + | + | + (0.108) |
| R3 | - | + (0.004) | + | + | + (0.122) |
| R4 | - | + (0.013) | + | + | + (0.035) |
| R5 | - | + (0.002) | + | + | + (0.053) |
| 729.HTLV-1ΔHBZ | |||||
| R6 | - | + (0.0003) | + | + | + (0.002) |
| R7 | - | + (0.0003) | + | + | + (0.004) |
| R8 | - | + (0.0008) | + | + | + (0.007) |
| R9 | - | + (0.0003) | + | + | + (0.001) |
| R10 | - | + (0.0002) | + | + | + (0.001) |
| R11 | - | + (0.0005) | + | + | + (0.013) |
| 729.HTLV-1HBZΔLZ | |||||
| R12 | - | + (0.0003) | + | + | + (0.001) |
| R13 | - | + (0.0002) | + | + | + (0.005) |
| R14 | - | + (0.0002) | + | + | + (0.014) |
| R15 | - | + (0.0002) | + | + | + (0.006) |
| R16 | - | + (0.0003) | + | + | + (0.004) |
| R17 | - | + (0.0001) | + | + | + (0.003) |
| 729 | |||||
| R18 | - | - (0.0001) | - | - | - (0.0001) |
| Media | |||||
| R19 | - | - (0.0001) | - | - | - (0.0001) |
Genomic DNA was isolated from rabbit PBMCs (weeks 2 and 8 after inoculation) and subjected to real-time Taqman PCR using HTLV-1-specific primers (670/671). Numbers in parentheses at weeks 2 and 8 denote copy number per cell in rabbit PBMCs.
- indicates no amplified PCR fragment; +, amplified PCR fragment.
*
Copy numbers in rabbits inoculated with the mutant proviruses at week 8 were significantly different from wild type, as determined by ANOVA followed by Tukey test (P < .001).