Figure 4.

PI3K inhibitors reproduced the inhibitory effect of PTEN on CXCR4-mediated chemotaxis. PIJ-17 Jurkat cells were cultured with or without dox (1 μg/mL) and were then pretreated with wortmannin (A) or LY294002 (B) for 1 hour at RT at the indicated concentrations. The cells were then subjected to chemotaxis assays with SDF-1α (100 ng/mL). The basal migration in the absence of SDF-1α was indicated as medium only. The results were representative of 4 independent experiments. Data shown are means ± SD.