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. 2005 Jul 7;106(9):3300–3307. doi: 10.1182/blood-2005-04-1632

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© 2005 by The American Society of Hematology

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Figure 1. — Functional expression of CCR5 on T_regs. (A) QPCR assessment of CCR5 expression in column-purified T CD25^- T cells from WT (▪) and CCR5^-/- () mice. Shown is CCR5 expression in freshly isolated cells, and cells cultured as in “Materials and methods.” Data are mean ± the standard error of the mean (SEM). (B) WT T_regs (▪) and CD4⁺CD25^- T cells () were cultured as in panel A, and migration in response to 10 ng/mL CCL3, CCL4, and CCL5 was determined. Data are mean ± SEM. ^*P < .05. (C) Chemotaxis of WT (▪) and CCR5^-/- () T_regs and CD4⁺CD25^- T cells, cultured as above, in response to 100 ng/mL CCL4. Data are mean ± SEM. (D) QPCR analysis of FoxP3 mRNA expression in cultured WT T_regs and CD4⁺ _regs and CD4⁺ CD25^- T cells prior to chemotaxis and in cells that migrated in response to 100 ng/mL CCL4. Data are mean ± SEM.

Inline graphic — Functional expression of CCR5 on T_regs. (A) QPCR assessment of CCR5 expression in column-purified T CD25^- T cells from WT (▪) and CCR5^-/- () mice. Shown is CCR5 expression in freshly isolated cells, and cells cultured as in “Materials and methods.” Data are mean ± the standard error of the mean (SEM). (B) WT T_regs (▪) and CD4⁺CD25^- T cells () were cultured as in panel A, and migration in response to 10 ng/mL CCL3, CCL4, and CCL5 was determined. Data are mean ± SEM. ^*P < .05. (C) Chemotaxis of WT (▪) and CCR5^-/- () T_regs and CD4⁺CD25^- T cells, cultured as above, in response to 100 ng/mL CCL4. Data are mean ± SEM. (D) QPCR analysis of FoxP3 mRNA expression in cultured WT T_regs and CD4⁺ _regs and CD4⁺ CD25^- T cells prior to chemotaxis and in cells that migrated in response to 100 ng/mL CCL4. Data are mean ± SEM.