Table 4.
TBI-induced fold changes in CDKIs' mRNA expression in BM-MNCs
|
Days after TBI
|
||||||
|---|---|---|---|---|---|---|
| 1 | 3 | 7 | 14 | 28 | 56 | |
| p21Cip1/Waf1 | 16.55 ± 4.55 | 10.37 ± 0.62 | 8.15 ± 1.65 | 3.60 ± 1.08 | 2.10 ± 0.40 | 1.38 ± 0.15 |
| p27Kip1 | –2.75 ± 0.25 | –4.15 ± 1.15 | –1.20 ± 0.10 | 1.15 ± 0.05 | 1.70 ± 0.20 | 1.47 ± 0.15 |
| p16Ink4a | 4.27 ± 1.33 | 9.59 ± 2.21 | 4.94 ± 0.77 | 10.54 ± 2.60 | 5.76 ± 1.44 | 4.63 ± 2.02 |
| p19Arf | –3.83 ± 0.67 | 0.52 ± 0.58 | 4.60 ± 0.52 | 8.23 ± 2.72 | 4.28 ± 0.93 | 4.21 ± 0.66 |
| p18Ink4c | –7.20 ± 0.30 | –5.00 ± 1.50 | 1.10 ± 0.10 | 1.00 ± 0.10 | 1.15 ± 0.05 | 1.35 ± 0.05 |
Mice were exposed to 6.5 Gy TBI or unirradiated as control. At 1, 3, 7, 14, 28, and 56 days after TBI, BM-MNCs were isolated from irradiated and unirradiated mice. RNA was extracted from these cells and analyzed by real-time RT-PCR. The relative quantity of individual CDKIs' mRNA was calculated for each sample after normalization with the expression of B2m mRNA and is presented as fold changes from control value ± SE (n = 3-8).