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. 2005 Dec 6;107(7):2736–2744. doi: 10.1182/blood-2004-07-2698

Figure 1.

Figure 1.

PAR2-AP and cAMP stimulation of release of VWF is delayed and P-selectin is reduced compared with PAR1-AP and thrombin. (A-B). Time course of VWF release in HUVECs: PAR2-AP, forskolin, and isoproterenol-mediated release of VWF is delayed compared with thrombin, PAR1-AP, or histamine. HUVECs were stimulated with thrombin (10 nM), PAR1-AP (100 μM), PAR2-AP (200 μM), histamine (20 μM), forskolin (20 μM with 100 μM IBMX), or isoproterenol (10 μM with 100 μM IBMX), and medium was collected at indicated time points. Data were corrected for the constitutive release of VWF and normalized to percentage of forskolin at 60 minutes, which consistently resulted in the highest levels of VWF. Values represent mean ± SEM. Results shown represent an experiment that was conducted 3 times. (C-D). Time course of cell-surface P-selectin expression in HUVECs: PAR2-AP– and forskolin-mediated P-selectin cell-surface expression is less when compared with PAR1-AP, thrombin, and histamine. HUVECs were stimulated with the above-mentioned concentrations of agonist, and the cell surface was assayed for P-selectin at indicated time points. Values represent mean ± SEM of an experiment that was conducted 3 times.