Figure 4.

MLR potential of DCs incubated with CD46-activated T-cell supernatant derived from a CD40L-deficient patient. (A) iDCs were incubated with supernatants derived from CD4⁺ T cells from a CD40L-deficient patient activated with CD3/CD46 or CD3/CD28, with or without the addition of a function neutralizing mAb to IL-10. The capacity of DCs to induce allogeneic T-cell activation after LPS addition was analyzed in an MLR. Shown is 1 representative of 3 independently performed experiments (with each activation condition in triplicate). (B) Summary and statistical evaluation of all proliferation data obtained in this set of experiments, displayed as described in Figure 1E.