Table 1.
IL-3 rescue assay
|
IC50, nM
|
|||
|---|---|---|---|
| Drug | Without IL-3 | With IL-3 | +IL-3/-IL-3 |
| CEP-701 | 8 | 24 | 3 |
| PKC412 | 29 | 155 | 5.3 |
| CGP52421 | 613 | 1053 | 1.7 |
BaF3/ITD cells were seeded onto 96-well plates containing increasing concentrations of CEP-701, PKC412, and CGP52421, both in the presence and absence of 1 ng/mL IL-3. MTT assay was performed at 48 hours, and the results were plotted as percent untreated control. Regression analysis of the resulting curves yielded the displayed IC50 values.
+IL3/-IL3 refers to the ratio of the IC50 values obtained in the presence and absence of IL-3