Figure 3.

Evaluation of IFN-γ on primitive and mature myeloid progenitor numbers isolated from the BM of Fanca^–/–, Fancg^–/–, and Fancc^–/– mice. (A) Evaluation of myeloid progenitors from WT, Fancc^–/– , Fanca^–/–, and Fancg^–/– bone marrow. Hematopoietic cells derived from the bone marrow LDMNCs from mice of each respective genotype were cultured in triplicate to evaluate growth of high proliferating potential colony-forming cells (HPP-CFCs) and low proliferating potential colony-forming cells (LPP-CFCs). Data represent the mean ± standard error of the mean (SEM) of 3 independent experiments. (B) Bone marrow cells from WT, Fancc^–/–, Fanca^–/–, and Fancg^–/– mice previously treated with a 7-day course of 400 μg/kg per day IFN-γ or vehicle control were cultured at 2 × 10⁴ BM LDMNCs/mL to determine the reduction in the respective populations of progenitors. Data represent the mean ± standard error of the mean (SEM) of 3 independent experiments.