Table 1.

Hematologic parameters in control and experimental mice before (baseline) and 3 weeks after treatment with pI-pC

Genotype	No.	Hgb level, g/L	MCV, fL	RDW, %	Reticulocytes, %	Spleen weight, %
Before pl-pC
WT	12	165 ± 4	55.3 ± 0.4	19.7 ± 0.2	2.6 ± 0.2	—
Rac1flox/flox	6	164 ± 3	51.8 ± 0.7	19.6 ± 0.4	2.9 ± 0.3	—
Rac2-/-	6	153 ± 4	50.4 ± 0.5a	21.3 ± 0.4b	3.4 ± 0.2	—
Rac1flox/flox; Rac2-/-	12	154 ± 3	48.8 ± 0.7c	20.4 ± 0.3	3.1 ± 0.2	—
After pl-pC
WT	12	160 ± 3	56.9 ± 1.0	18.6 ± 0.6	3.8 ± 0.3d	0.36 ± 0.03
Rac1-/-	6	155 ± 2e	54.2 ± 0.5e	21.2 ± 0.7	3.9 ± 0.2e	0.43 ± 0.07
Rac2-/-	6	142 ± 5f	49.6 ± 0.9g	21.5 ± 0.7	4.8 ± 0.5h	0.37 ± 0.03
Rac1-/-; Rac2-/-	12	128 ± 3ij	43.3 ± 0.6ij	28.1 ± 1.4ij	7.2 ± 0.4ij	0.72 ± 0.15k

All values are mean ± SEM. Population is 12 for the groups of WT and Mx1Cre^Tg/+; Rac1^flox/flox; Rac2^-/- mice and 6 for the groups of Mx1Cre^Tg/+; Rac1^flox/flox and Rac2^-/- mice. Corresponding Rac1 protein levels by densitometry after treatment with pl-pC, at the time these blood counts were obtained, were 99.9 ± 1.8% for the WT mice and 42.3 ± 3.5% (range, 25.6%-56.8%) for the Rac1^-/-; Rac2^-/- mice. Spleen weight was evaluated for 6 mice per group and is expressed as percent of the body weight.

To convert hemoglobin level from grams per liter to grams per deciliter, divide grams per liter by 10.

— indicates not determined. Spleen size within normal limits was observed for all genotypes before pl-pC treatment.

^aP < .05 versus WT at baseline.

^bP < .05 versus WT and Rac1^-/- at baseline.

^cP < .05 versus WT and Rac1^-/- at baseline.

^dP < .05 versus WT at baseline.

^eP < .05 versus Rac1^flox/flox at baseline.

^fP < .05 versus WT after pl-pC treatment.

^gP < .05 versus WT and Rac1^-/- after pl-pC treatment.

^hP < .05 versus Rac2^-/- at baseline.

ⁱP < .05 versus WT, Rac1^-/-, and Rac2^-/- after pl-pC treatment.

^jP < .001 versus Rac1^flox/flox; Rac2^-/- at baseline.

^kP < .05 versus WT after pl-pC treatment.