Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Detection of ubiquitinated Stat5A in the nucleus. 32D (EpoR wt) cells were starved overnight, followed by stimulation with IL-3 in the presence or absence of MG132. Cytoplasmic and nuclear extracts were prepared from these cells. Subsequently, active Stat5 was pulled down from the extracts using the biotin-labeled double-stranded Stat5-binding oligodeoxynucleotides and subjected to Western blot analyses with antiubiquitin (α-Ub) or anti-Stat5A (α-Stat5A) antibodies.
