Figure 1.

Expression of cyclin D1 isoforms by cultured MCL cells. Four MCL cell lines (Jeko-1, SP53, SP49, and Mino) and the control, germinal center-derived diffuse large B-cell lymphoma cell line (VAL) were examined for expression of cyclin D1 protein isoforms using commercially available antibodies against cyclin D1a (A) and cyclin D1, which was not further characterized (B), and 2 different custom-made, cyclin D1b-specific antibodies (C⁴, D⁷). Bands corresponding to cyclin D1a (37 kDa) and cyclin D1b (33 kDa) are marked by the open and solid arrowheads, respectively. Results are representative of 2 to 4 independent experiments.