Table 1.

Conserved element mutant summary

CE	Sequence	Mutation
1	AAAAGGGGG	AAAAGGGcc
2	TGACATC	acACATC
3	CAGATGGCTG	gtGATGGCTG
4	TGCCCCACC	TGCCaaACC
6	GTGACACAACCCTGTTCCCTGTC	GacACACAACCCTGTTCCCTGTC
7	GTTCCCGCTTATCTC	GTTaaCGCTTATCTC
9	TTCTTGGAAATGAG	ggaTTGGAAATGAG
10	AGAGCAAAGGGGAGG	AGAGCAAAGccaAGG

Wild-type sequences for conserved elements in the hepcidin promoter are listed. To disrupt putative transcription factor binding sites, mutations in conserved elements (lower-case letters) were introduced individually into the hepcidin 0.6 kb-luc (mini) plasmid by site-directed mutagenesis.