Table 1.

Overview of experiments performed in this study

Vector, MOI	Titer of supernatant, 106/mL	Treated cells	Positive wells on 96-well plate	Frequency of replating cells	No. clones of analyzed samples	Mean copy no., d 7 qPCR
Pilot experiment
LTRSFdsRED, 5	3	1 × 106	56; 81	1 in 80	1 ; 3	ND
Experiment 1
LTRSFeGFP, 20	15	1 × 105	50; 70	1 in 102	2 ; 9	ND
SINSFeGFP, 20	5	1 × 105	2; 3	1 in 3790	ND	ND
Mock, 0	—	1 × 105	4; 5	< 1 in 2083	ND	ND
Experiment 2
LTRSFeGFP, 20	20	1 × 105	96; 96	> 1 in 22	2 ; 4	11.6
SINSFeGFP, 20	5	1 × 105	0; 6	1 in 3150	ND	2.9
LTRSFdsRed, 20	23	1 × 105	96; 96	> 1 in 22	2 ; 4	13.9
SINSFdsRed, 20	5	1 × 105	0; 0	< 1 in 9550	ND	5.4
Mock, 0	—	1 × 105	0	< 1 in 9550	ND	ND
Experiment 3
LTRSFeGFP, 20	8	1 × 105	52; 40	1 in 153	1 ; 10	3
SINSFeGFP, 20	9	1 × 105	1; 0	< 1 in 9550	ND	3.1
Mock, 0	—	1 × 105	0; 0	< 1 in 9550	ND	ND
Experiment 4
LTRSFeGFP, 20	9	1 × 105	42; 40	1 in 180	3 ; 3	6.4
LTRSFeGFP, 10	9	1 × 105	20	1 in 428	ND	4.2
LTRSFeGFP, 5	9	1 × 105	15	1 in 589	ND	2.2
LTRSFeGFP, 2.5	9	1 × 105	15	1 in 589	ND	0.9
SINSFeGFP, 20	10	1 × 105	32; 34	1 in 237	2 ; 6	4.4
Mock, 0	—	1 × 105	0	< 1 in 9550	ND	ND
Experiment 5
LTRSFeGFP, 20	10	1 × 105	96	> 1 in 22	ND	4
LTRSFeGFP, 5	10	1 × 105	31	1 in 256	ND	1.5
LTRSFeGFP, 1.25	10	1 × 105	0	< 1 in 9550	ND	0.6
SINSFeGFP, 20	10	1 × 105	13	1 in 687	1 ; 5	1.7
SINSFeGFP, 5	10	1 × 105	0	< 1 in 9550	ND	0.6
SINSFeGFP, 1.25	10	1 × 105	0	< 1 in 9550	ND	0.5
Mock	—	1 × 105	0	< 1 in 9550	ND	ND

Titer was determined by limiting dilution on SC-1 fibroblasts and was adjusted before the transduction ; bone marrow cells. Pairs of numbers separated by semicolons result from duplicate determinations

ND indicates not determined; —, not applicable