Figure 6.

AMA1 is required for meiotic development. (A) Ama1p is required for meiosis I. Wild type (WT), ama1Δ, or cdc16-1 diploids were induced to enter meiosis and timepoints taken as indicated (h). The nuclei and spindles were visualized by DAPI staining or by indirect immunofluorescence using Tub1p antibodies, respectively (×1,000 final magnification). (B) Ama1p is not required for meiosis II. The spo13 single or ama1Δ spo13 double mutant was induced to enter meiosis then harvested after 24 h. Dyads (two spored asci) in the spo13 mutant (Upper) were visualized by Nomarski optics (Nom) or DAPI staining (arrows). Binucleated ama1 spo13 cells were identified by DAPI staining (arrows, Lower). (C) The ama1 spo13 mutant performs a single equational division. One homolog of chromosome V was marked with a tandem array of the Tet repressor. This chromosome was visualized by expressing the Tet repressor-GFP fusion protein and fluorescent microscopy.