Table 1.
PCR detection of HTLV-1 proviral sequences in inoculated rabbit PBMCs
|
Inoculum and rabbits
|
Weeks after inoculation*
|
|||
|---|---|---|---|---|
| 2 | 4 | 6 | 8 | |
| HTLV-1 | ||||
| R1 | + | + | + | + |
| R2 | + | + | + | + |
| R3 | + | + | + | + |
| R4 | + | + | + | + |
| R5 | + | + | + | + |
| HTLV-IΔPBM | ||||
| R39 | – | – | – | – |
| R40 | – | + | – | – |
| R41 | – | + | + | – |
| R42 | – | – | – | – |
| R43 | – | – | – | – |
| 729 control | ||||
| R18 | – | – | – | – |
Genomic DNA was isolated from rabbit PBMCs and subjected to PCR using HTLV-1–specific primers (670-671).
+ indicates that HTLV-1 proviral sequences were detected by PCR in inoculated rabbit PBMCs; –, that HTLV-1 proviral sequences were not detected.
*
All rabbits were negative at week 0