Table 2.

Primers for real-time PCR analysis of ChIP material

Target region	Central CACC site sequence	Product size, bp, or reference	Primer sequences
β-Major globin	CCACACCCT	Letting et al37	Forward: 5′-GAC AAA CAT TAT TCA GAG GGA GTA CCC
Reverse: 5′-AGG TGC ACC ATG ATG TAT GTT TAT GG
Nectin	N/A	Letting et al37	Forward: 5′-TTT ACA TAA GCC TAG TGG TAC CCT
Reverse: 5′-ATC GCT GTC CTG CAT CTC ACA GTC G
ECR a	TGGGTGCGGC	139	Forward: 5′-AAT GAG AGG GTG GGT GGT TT
Reverse: 5′-CGG AAG ACC CCC TTC AAC
ECR 1	CCCCACCCT	223	Forward: 5′-CTT GGT GGC CTG AGA AAA GG
Reverse: 5′-ACA GGC TCC AGC TAG GTC AC
ECR 2	AGGGAGAGT	205	Forward: 5′-CAT GCC AGG GAG CTG TGA
Reverse: 5′-AGC TCT GCC CAA CAC ATG G
ECR 3 (1&2)*	1-TCCCACCCC	140	Forward: 5′-AGG GAG ATC AGA CCG CTG AG
2-GCCCACCCA	Reverse: 5′-GAG GCA GCT TCT CCA GGA AC
ECR 3 (3)*	AGGGTGGGG	185	Forward: 5′-ACT CGG ACT CCC CAG CTC
Reverse: 5′-GAA GGA GGG GAG CTG GTC
ECR 3 (4)*	GCCCGCCCT	103	Forward: 5′-CGC CAC TAT CAG TTG TTG G
Reverse: 5′-CAG TCC CCT GCT GCT CTG
ECR 6	CCCCTCCCC	179	Forward: 5′-CCC TTC CTG TCT CCT CCA GT
Reverse: 5′-CTA TTG GGG CAA AGC TAG GA
ECR 7	AGGGTGGGG	169	Forward: 5′-CTT GGT CTT CCA GAG GGA TG
Reverse: 5′-AGC CTT GGA GGA GGA GCT A
ECR 8 (1)*	CCACGCCCT	169	Forward: 5′-GGA GAG GAG ATG CGC TGA
Reverse: 5′-CTA TGA GGC AGG GGA AGA GA
ECR 8 (2)*	CCCCTCCCT	182	Forward: 5′-GCA CTC CTG TAG CCT ATC CTG
Reverse: 5′-TGC CAA GAC AAC CTC CTG
ECR 10	AGGGTGGGG	196	Forward: 5′-GTC CAG GCT CAA CCT TTC C
Reverse: 5′-TGG GCT TCG TTC TCC ATA AA
ECR 12 (1)*	CCCCTCCCTC	144	Forward: 5′-GCT GGA GAC AGC TTC AAA GG
Reverse: 5′-GCA AGA AAG GAA ACA GTG AGG
ECR 12 (2&3)*	2-CCCCTCCCT	162	Forward: 5′-CCT CCG ATT ATT GCA GGA
3-GCCCACCCC	Reverse: 5′-CCG AGA AAG GCC TGA GAA G
ECR 12 (4)*	CGGGAGGGT	141	Forward: 5′-CCA TCA CAA AGA GCC TTA ATC C
			Reverse: 5′-GGG TGT TGC CCC AGT TTT A

^*Parenthetic values indicate CACC sites within the ECR