Table 2.
Percentage of leukocyte subsets from platelet-depleted and control mice following thermal injury
|
Animal group, by time following injury
|
Percent leukocyte subset, × 109/L, mean ± SEM
|
|||
|---|---|---|---|---|
| No. mice* | Lymphocytes | Monocytes | Neutrophils | |
| 24 h after injury | ||||
| CTL-Ig Sham | 8 | 85.75 ± 1.59 | 4.15 ± 0.31 | 10.38 ± 1.40 |
| CTL-Ig Burn | 7 | 68.19 ± 2.48 | 9.68 ± 1.06 | 24.60 ± 3.76 |
| αPLT-Ig Sham | 8 | 87.37 ± 1.54 | 3.92 ± 0.30 | 8.00 ± 1.32 |
| αPLT-Ig Burn | 10 | 75.05 ± 2.30 | 8.64 ± 0.67 | 16.92 ± 2.03 |
| 36 h after injury | ||||
| CTL-Ig Sham | 3 | 85.37 ± 0.50 | 3.28 ± 0.04 | 9.02 ± 0.31 |
| CTL-Ig Burn | 4 | 74.08 ± 2.06 | 9.21 ± 0.53 | 14.91 ± 1.78 |
| αPLT-Ig Sham | 4 | 88.67 ± 0.90 | 3.03 ± 0.37 | 6.63 ± 0.48 |
| αPLT-Ig Burn | 9 | 66.10 ± 1.48† | 13.19 ± 1.11‡ | 20.35 ± 1.64† |
| 48 h after injury | ||||
| CTL-Ig Sham | 8 | 86.00 ± 1.51 | 4.43 ± 0.22 | 8.21 ± 1.18 |
| CTL-Ig Burn | 7 | 70.86 ± 1.69 | 6.18 ± 0.78 | 22.10 ± 1.93 |
| αPLT-Ig Sham | 7 | 88.18 ± 1.42 | 4.85 ± 0.36 | 6.26 ± 1.07 |
| αPLT-Ig Burn | 5 | 73.24 ± 1.06 | 13.02 ± 0.78§ | 6.11 ± 0.47‡ |
Anticoagulated whole blood from experimental or control mice was analyzed for percent lymphocytes, monocytes, and neutrophils at the times listed. Mice per group per time point are listed. Statistical significance of the data (mean ± SEM) was analyzed using the Mann-Whitney test.
*
Animals per group from 3 independent experiments.
†
P < .05 for CTL-Ig Burn compared with αPLT-Ig Burn.
‡
P < .005 for CTL-Ig Burn compared with αPLT-Ig Burn.
§
P < .001 for CTL-Ig Burn compared with αPLT-Ig Burn.