Figure 3.
WT1 (–Ex5/–KTS) binds to the A1 promoter. Chromatin immunoprecipitation assays were performed as described using 32DV4 and 32DWT1 cells treated overnight with 50 μM ZnCl2. The arrow indicates the position of the PCR product representing the A1 promoter, present in the 32DWT1 sample but not in the 32DV4 sample. Lane 1 is the control with no template, lanes 2 and 5 are the no-antibody controls from 32DV4 and 32DWT1 cells, respectively, and lanes 3 and 4 are the samples from 32DV4 cells (Lane 3) and 32DWT1 cells (Lane 4). The identity of the PCR fragment was confirmed by sequencing.