Table 1.

IFNγ levels in APC cultures with MSCs pretreated with anti-IFNγRI

Cells/pretreated MSCs	IFNγ, pg/mL
Unpulsed MSCs
Isotype	20 ± 4
Anti-IFNγRI	22 ± 2
Pulsed MSCs
Isotype	120 ± 8
Anti-IFNγRI	30 ± 3
Activated CD4+
None	88 ± 7
Unactivated CD4+
None	5 ± 0.5
Unpulsed MSCs + unactivated CD4+
Isotype	18 ± 3
Anti-IFNγRI	15 ± 2
Unpulsed MSCs + activated CD4+
Isotype	92 ± 5
Anti-IFNγRI	78 ± 5
Pulsed MSCs + unactivated CD4+
Isotype	160 ± 5
Anti-IFNγRI	28 ± 3
Pulsed MSCs + activated CD4+
Isotype	385 ± 12*
Anti-IFNγRI	60 ± 6*

MSCs from 4 different donors were subjected to overnight incubation with 0.1 to 1 μg/mL IFNγRI. Cells were washed and then pulsed with C albicans or T toxoid. After this, the MSCs were washed and then placed in APC cultures in the presence of anti-IFNγRI (0.1-1 μg/mL) or isotype control. After 24 hours, culture supernatants were quantitated for IFNγ by ELISA. The data are shown for values at 1 μg/mL anti-IFNγRI.

^*These values showed significant (P < .05) differences between anti-IFNγRI and isotype control.