Table 1.

Kinases identified in the Bcr-Abl–containing and control cell lines

PSDB ID	pTyr Site	Peptide	BV173	K562	KCL22	KU812	SD1	SUP-B15	U937	Identified in K562 IMAC*	Novel site
Lipid kinase
PIK3R1	580	DQyLMWLTQK	+	-	-	-	-	+	-	-	-
PIK3R2	467	EYDQLYEEyTR	+	-	-	-	-	-	-	+
Protein kinase
Abl	115	NGQGWVPSNyITPVNSLEK	+	+	+	+	-	+	-	-	-
Abl	185	INTASDGKLyVSSESR	+	+	+	+	+	+	-	+	-
Abl	226	NKPTVyGVSPNYDK	+	+	+	+	+	+	-	-	-
Abl	253	LGGGQyGEVYEGVWK	-	+	-	-	-	-	-	+	-
Abl	257	LGGGQYGEVyEGVWK	-	+	+	-	-	+	-	+	-
Abl	393	LMTGDTyTAHAGAK	+	+	+	+	+	+	-	+	-
Ack	596	KPTyDPVSEDQDPLSSDFKR	-	-	-	-	+	-	-	-	-
Bcr	177	GHGQPGADAEKPFyVNVEFHHER	+	+	+	+	+	+	-	+	-
Bcr	246	SSESSCGVDGDyEDAELNPR	-	+	-	-	-	-	-	-	-
Bcr	436	KTGQIWPNDGEGAFHGDADGSFGTPPGyGCAADR	-	+	-	-	-	-	-	-	+
Bcr	591	LASQLGVyR	-	+	-	-	-	-	-	+	-
Bcr	598	AFVDNyGVAMEMAEK	-	+	-	-	-	-	-	-	+
Bcr	644	NSLETLLyKPVDR, NSLETLLyKPVDRVTR	+	+	+	+	-	-	-	+	-
Btk	223	KVVALyDYMPMNANDLQLR	+	+	-	-	-	+	-	-	-
Btk	225	KVVALYDyMPMNANDLQLR	+	-	-	-	-	-	-	-	+
Btk	361	HLFSTIPELINyHQHNSAGLISR	+	-	-	-	-	-	-	-	+
Cdc2	15	IEKIGEGTyGVVYK	+	+	+	+	+	+	+	-	-
Cdc2	19	IGEGTYGVVyKGR	-	+	-	-	+	-	-	-	-
Cdk2	15	VEKIGEGTyGVVYK	+	+	+	+	+	+	+	-	+
Cdk2	15	IGEGTyGTVFK	+	-	-	-	-	-	-	-	-
DYRK1A	145	KVYNDGYDDDNyDYIVK	-	+	+	-	-	+	-	-	-
DYRK1A	319	IyQYIQSR	-	-	-	-	-	-	+	-	+
DYRK1A	321	IYQyIQSR	+	+	+	+	+	+	+	-	-
DYRK2	309	VYTyIQSR	+	-	-	-	-	-	+	-	+
ERK1	204	IADPEHDHTGFLTEyVATR	+	+	+	+	+	+	+	-	-
ERK2	187	VADPDHDHTGFLTEyVATR	+	+	+	+	+	+	+	-	-
Fyn	214	KLDNGGYyITTR	-	+	-	-	-	-	-	-	+
Fyn	420	LIEDNEyTAR	+	-	-	-	+	-	-	-	-
GSK3-β	216	GEPNVSyICSR	+	+	+	+	+	+	+	-	-
Hck	209	TLDNGGFyISPR	-	-	-	-	-	-	+	-	+
Hck	411	VIEDNEyTAR	+	-	-	-	-	-	-	-	-
HIPK1	352	AVCSTyLQSR	+	+	+	+	+	+	+	-	-
HIPK3	359	TVCSTyLQSR	+	-	+	-	-	+	-	-	+
IGF1R	1189	DIYETDyYRK	-	-	-	-	+	-	-	-	-
Lck	192	NLDNGGFyISPR	-	-	-	-	+	-	-	-	-
Lck	505	SVLEDFFTATEGQyQPQP	-	+	-	-	+	+	-	-	-
Lyn	193	SLDNGGyYISPR	-	+	-	-	-	-	-	-	+
Lyn	508	AEERPTFDYLQSVLDDFYTATEGQyQQQP	+	-	-	-	-	+	+	-	-
P38-α	182	HTDDEMTGyVATR	-	+	+	+	+	+	+	-	-
PKCD	313	SDSASSEPVGIyQGFEK	+	-	-	-	-	-	-	-	-
PRP4	849	LCDFGSASHVADNDITPyLVSR	+	+	+	+	+	+	+	-	-
SgK223	390	CLGLTGEPQPPAHPQEATQPEPIyAESTK	+	-	-	-	-	+	-	-	-
Syk	323	QESTVSFNPyEPELAPWAADKGPQR	-	-	-	-	+	-	-	-	-
Syk	348	EALPMDTEVyESPyADPEEIRPK	-	-	-	-	+	-	-	+	-
Syk	352	EALPMDTEVYESPyADPEEIRPK	-	-	-	-	+	-	-	+	-

For a complete listing of phosphotyrosine peptides found in these samples, see Table S1. Bcr-Abl—containing cell lines were BV173, K562, KCL22, KU812, SD1, and SUP-B15. Control cell line was U937. PSB ID is a protein identifier referenced from PhosphoSite.²¹

^*Sites identified in K562 IMAC appear in Salomon et al.⁴⁰