Table 5.
Kinases identified in the FIP1L1-PDGFRα cell line EoL-1
| PSDB ID | pTyr sites | Peptide | Novel site |
|---|---|---|---|
| Lipid kinase | |||
| PIK3CB | 772 | EALSDLQSPLNPCVILSELyVEK | Yes |
| PIK3CD | 440 | CLYyMWPSVPDEKGELLNPTGTVR | Yes |
| PIK3CD | 485 | SNPNTDSAAALLICLPEVAPHPVYyPALEK | Yes |
| PIK3CD | 524 | GSGELyEHEKDLVWK | Yes |
| PIK3R1 | 580 | DQyLMWLTQK | No |
| Protein kinase | |||
| Ack | 518 | KPTyDPVSEDQDPLSSDFKR | No |
| Cdc2 | 15 | IEKIGEGTyGVVYK | No |
| Cdc2 | 19 | IEKIGEGTYGVVyK | No |
| DYRK1A | 321 | IYQyIQSR | No |
| ERK1 | 204 | IADPEHDHTGFLTEyVATR | No |
| ERK2 | 187 | VADPDHDHTGFLTEyVATR | No |
| FIP1L1* | 95 | TGAPQyGSYGTAPVNLNIK | Yes |
| FIP1L1* | 98 | TGAPQYGSyGTAPVNLNIK | Yes |
| GSK3-β | 216 | GEPNVSyICSR | No |
| Hck | 411 | VIEDNEyTAR | No |
| Hck | 522 | NRPEERPTFEYIQSVLDDFYTATESQyQQQP | No |
| Lyn | 507 | EKAEERPTFDYLQSVLDDFYTATEGQyQQQP | No |
| PDGFRα | 742 | QADTTQyVPMLER | No |
| PDGFRα | 762, 768 | SLyDRPASyK | No |
| PDGFRα | 1018 | LSADSGyIIPLPDIDPVPEEEDLGKR | No |
| PRP4 | 849 | LCDFGSASHVADNDITPyLVSR | No |
| Syk | 323 | QESTVSFNPyEPELAPWAADKGPQR | No |
For a complete listing of phosphotyrosine peptides found in this sample, see Table S2.
*
Fusion partner with PDGFRα.