Table 2.

Quantification of intracellular λ light chain retained in treated myeloma cell lines

	Experiment 1		Experiment 2		Experiment 3
Ratio	8226/S	MM.1S	8226/S	MM.1S	8226/S	MM.1S
BZ/UNTX	0.257	0.854	0.481	1.387	0.304	1.316
BfA/UNTX	2.208	1.517	1.908	1.470	2.603	1.402

The 8226/S and MM.1S myeloma cell lines were left untreated, treated with 10 (MM.1S) to 100 nM (8226/S) bortezomib (BZ), or treated with 1 μM brefeldin A (BfA). Cell lysates were prepared after 7 hours of treatment, and 500 ng total protein was used to determine the amount of intracellular λ light chain present by ELISA. Three independent experiments are shown with the data presented as the ratio of the treated cells (BZ or BfA) to the untreated controls.