Table 2.
Lymphoid and myeloid population in hematopoietic organs of Hoxb4 mutants and control littermates
|
Hoxb4−/−, %
|
Control, %
|
|||||
|---|---|---|---|---|---|---|
| BM | Spleen | Thymus | BM | Spleen | Thymus | |
| B cells | ||||||
| B220+/CD43 | 24 ± 3.7 | 36 ± 13 | — | 30 ± 1.8 | 55 ± 4.8 | — |
| B220+/CD43+ | 3.0 ± 0.2 | 8.4 ± 1.6 | — | 4.1 ± 0.8 | 6.9 ± 0.7 | — |
| B220+/lgM+ | 8.0 ± 3.3 | 49 ± 13 | — | 15 ± 1.5 | 56 ± 3.7 | — |
| B220+/lgM− | 17 ± 1.0 | 2.7 ± 19 | — | 17 ± 0.9 | 6.1 ± 2.2 | — |
| T cells | ||||||
| CD4 | — | 26 ± 5.1 | 6.9 ± 1.3 | — | 20 ± 1.1 | 6.6 ± 0.5 |
| CD8 | — | 17 ± 3.5 | 7.2 ± 0.8 | — | 12 ± 0.9 | 4.4 ± 0.4 |
| CD4/CD8 | — | 0 | 84 ± 2.2 | — | < 0.01 | 87 ± 0.9 |
| Myeloid cells | ||||||
| Mac1 | 17 ± 1.3 | 5.6 ± 1.6 | — | 18.6 ± 1.1 | 5.4 ± 0.8 | — |
| Mac1/Gr1 | 11 ± 1.8 | 0.8 ± 0.5 | — | 9.2 ± 1.2 | 0.4 ± 0.1 | — |
Percentages of lymphoid and myeloid cell populations in BM, spleen, and thymus of mutant and control animals using FACS analysis. Comparison between mutant and control cell populations and total cell numbers in Tables 1 and 2 was evaluated using a 2-tailed Student t test; P > .05 for all groups. n = 3 mice per group.
— indicates not done.