Table 1.
Down-regulation of splenic erythroblast cell-surface Fas/FasL in ts-VHL−/− mice
|
Subset frequency, % of Ter 119+ cells
|
Fas-positive cells, %
|
FasL-positive cells, %
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| Control*; n = 6 | ts-VHL −/−; n = 5 | P† | Control*; n = 6 | ts-VHL −/−; n = 5 | P† | Control*; n = 6 | ts-VHL −/−; n = 5 | P† | |
| Spleen | |||||||||
| ProE | 0.6 ± 0.2 | 1.5 ± 0.3 | — | 38 ± 9 | 9 ± 3 | .023 | 66 ± 4 | 36 ± 9 | .025 |
| Ery.A | 24 ± 5 | 36 ± 3 | .044 | 48 ± 6 | 26 ± 3 | .011 | 79 ± 2 | 60 ± 5 | .018 |
| Ery.B | 24 ± 4 | 39 ± 3 | .012 | 12 ± 1 | 3 ± 0.4 | < .001 | 37 ± 4 | 12 ± 4 | .002 |
| Ery.C | 52 ± 8 | 24 ± 4 | .045 | 0.6 ± 0.1 | 0.3 ± 0.1 | — | 1 ± 0.2 | 0.6 ± 0.2 | — |
| Non-Ery | NA | NA | — | 47 ± 4 | 47 ± 6 | — | 10 ± 0.2 | 9 ± 1 | — |
| Bone marrow | |||||||||
| ProE | 1.9 ± 0.3 | 2.0 ± 0.4 | — | 21 ± 3 | 13 ± 3 | — | 35 ± 4 | 28 ± 5 | — |
| Ery.A | 35 ± 4 | 35 ± 5 | — | 21 ± 2 | 17 ± 4 | — | 51 ± 3 | 41 ± 4 | — |
| Ery.B | 34 ± 2 | 25 ± 1 | .001 | 2 ± 0.5 | 1 ± 0.2 | — | 9 ± 2 | 6 ± 2 | — |
| Ery.C | 29 ± 4 | 38 ± 6 | — | 0.3 ± 0.05 | 0.3 ± 0.1 | — | 0.6 ± 0.1 | 0.3 ± 0.1 | — |
| Non-Ery | NA | NA | — | 51 ± 4 | 50 ± 3 | — | 15 ± 4 | 18 ± 5 | — |
Non-Ery indicates nonerythroid; NA, not applicable; and —, not significant.
*
Matched littermates, number of mice indicated (n).
†
Two-tailed Student t test, unequal variance, only for statistically significant differences at P < .05.