Table 3.

Quantitative real-time PCR for Fas, FasL, and FasB mRNAs in chronic erythropoietic stress

	Fas		FasL		FasB
Mouse strain*	ΔΔCT†	Fold decrease in mRNA‡	ΔΔCT†	Fold decrease in mRNA‡	ΔΔCT†	Fold decrease in mRNA‡
ts-VHL−/−
Experiment 1	1.12 ± 0.29	—	0.99 ± 0.42	—	1.12 ± 0.80	—
Experiment 2	2.61 ± 0.09	—	2.52 ± 0.17	—	2.64 ± 0.86	—
Experiment 3	2.05 ± 0.17	—	1.44 ± 0.16	—	2.51 ± 0.50	—
Experiment 4	1.00 ± 0.13	—	1.19 ± 0.07	—	0.49 ± 0.30	—
Mean	1.70 ± 0.38	3.2	1.54 ± 0.34	2.9	1.69 ± 0.52	3.2
β-thal
Experiment 1	1.75 ± 0.28	—	0.16 ± 0.66	—	2.29 ± 0.90	—
Experiment 2	3.56 ± 0.26	—	1.9 ± 0.24	—	4.3 ± 0.09	—
Experiment 3	2.35 ± 0.07	—	(−0.5) ± 0.47	—	2.14 ± 0.08	—
Mean	2.55 ± 0.53	5.9	0.52 ± 0.7	1.4	2.92 ± 0.70	7.6

^*RNA prepared and real-time PCR carried out independently for each experiment, from Ery.A sorted from at least one β-thal or ts-VHL ^−/− mouse and one matched littermate.

^†Mean ± SD of ΔΔCT from triplicate samples for each experiment. ΔΔCT is the difference in cycle number for the indicated mRNAs, between template RNA from β-thal or ts-VHL ^−/− mouse and its matched littermate, relative to β-actin mRNA in each sample.

^‡Calculated as 2 to the power of the mean ΔΔCT for all experiments.