Table 2.

Cross-competition experiments between polyclonal or monoclonal anti-CD62 antibody and O157LPS

	Detection of O157LPS binding			Detection of anti-CD62 binding
	OL157 LPS without anti-CD62	Simultaneous incubation of anti-CD62 and LPS	P	CD62 expression without OL157 LPS	Simultaneous incubation of anti-CD62 and LPS	P
Polyclonal α-CD62	91.0 (84.3-95.1)*	3.0 (1.8-3.7)	.008	87.0 (82.7-97.3)	6.5 (4.9-12.2)	.008
Monoclonal α-CD62	77.1 (51.3-99.9)*	69.4 (47.4-91.2)	.548†	53.2 (47.9-61.5)	4.0 (1.0-5.2)	.008

Washed thrombin-stimulated platelets were incubated with O157LPS (1 μg/mL, diluted in donor plasma) or anti-human CD62 (rabbit or mouse) or were simultaneously incubated with O157LPS and anti-CD62 (diluted in donor plasma). Incubation of platelets with polyclonal anti-CD62 and LPS reduced LPS binding by 97% and CD62 expression by 93%, respectively. Simultaneous incubation of platelets with the monoclonal antibody and LPS reduced LPS binding minimally and CD62 expression by 92%.

^*Differences in baseline levels of anti-LPS binding because of donor platelet variations.

^†Not significant.