Table 3.

LPS binding to mouse platelets: the effect of anti-CD62 on binding

Strain	Median LPS binding without thrombin, % (range)	P	Median LPS binding with thrombin, % (range)	P	LPS binding to thrombin-activated WPs preincubated with 10 μg/mL anti-CD62, % (range)	LPS binding to thrombin-activated WPs preincubated with 50 μg/mL anti-CD62, % (range)
C57BL/10ScSn*	58.2 (30.5-70.3)	<.001	63.7 (30.4-77.5)	<.001	54.6 (40.0-67.2)	16.2 (6.9-26.6)
C57BL/10ScN*	0.7 (0.1-7.5)	—	40.5 (30.4-48.7)	—	36.3 (31.2-45.7)	NT
C3H/HeN†	71.3 (52.3-88.6)	.841‡	70.2 (58.6-89.9)	.055‡	51.6 (17.0-57.6)	7.1 (5.3-9.5)
C3H/HeJ†	61.9 (52.3-79.1)	—	58.8 (45.1-68.8)	—	33.7 (10.6-34.8)	NT

Blood was drawn through retro-orbital venous sampling into tubes containing 0.129 M sodium citrate. PRP was obtained by centrifugation at 270g for 3 minutes. Washed thrombin-stimulated or unstimulated platelets were diluted to 10⁸/mL in autologous plasma and incubated with O157LPS 1 μg/mL, fixed, washed, and incubated with monovalent O rabbit antiserum O157-O56. LPS binding to thrombin-activated washed platelets was decreased by preincubation of the platelets with anti-mouse CD62 antibody.

WPs indicates washed platelets; wt, wild-type;—, not applicable; and NT, not tested.

P values were evaluated by comparisons of platelets from wild-type mice and mutant mice.

^*n - 22 mice.

^†n = 31 mice.

^‡Not significant.