Table 6.

Expression CD40L on mouse platelets detected by flow cytometry

		CD40L expression
Mouse strain	No. mice	Resting platelets	Platelets incubated with O157LPS	P*	Thrombin-stimulated platelets	P*	Resting platelets preincubated with 10 μg/mL anti-CD62 and O157 LPS	P†	Resting platelets preincubated with 50 μg/mL anti-CD 62 and O157 LPS	P†
C57BL/10ScSn	10	1.7 (0.1-3.7)	45.4 (25.6-75.9)	.001	59.3 (42.6-72.6)	.053‡	39.8 (35.2-65.5)	.631‡	17.2 (9.1-39.4)	.001
C57BL/10ScN	5	4.0 (3.0-4.1)	0.14 (0.10-0.35)	NA	49.5 (45.9-65.8)	NA	1.04 (0.68-1.54)	.197‡	NA	NA
C3H/HeN	10	0.3 (0.2-1.1)	45.3 (25.6-81.6)	.001	54.5 (18.2-72.8)	.490‡	32.5 (17.6-49.2)	.393‡	21.2 (10.2-34.1)	.001
C3H/HeJ	5	0.3 (0.1-0.9)	0.99 (0.01-1.49)	NA	49.3 (30.7-63.0)	NA	0.29 (0.08-6.53)	.548‡	NA	NA

Expression of CD40L is shown as median and range values of experiments. To inhibit the CD62 phenotype, platelets were preincubated with rat anti-mouse CD62 antibody (10 μg/mL or 50 μg/mL) or rat IgG (at the same concentration), followed by incubation with O157LPS (diluted in autologous plasma).

NA indicates not analyzed.

^*P values evaluated by comparison of platelets from wild-type mice with those of mutated mice.

^†P values evaluated by comparison of resting platelets incubated with LPS or LPS and anti-CD62. Rat IgG did not block the CD62 phenotype (results not shown).

^‡Not significant.