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Growth rates of murine hematopoietic cells expressing FLT3-ITD Tyr→Phe mutants. Analysis of IL-3–independent growth in Ba/F3 (A) and 32D (B) cell lines stably transformed with each of the FLT3 mutants. Cells were washed twice in PBS, resuspended in RPMI and 10% FCS in the absence of IL-3, and seeded at a density of 1 × 105/mL. Viable cells were counted by trypan blue exclusion.
