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. 2005 Oct 18;107(3):1063–1069. doi: 10.1182/blood-2005-08-3123

Figure 3.

Figure 3.

Activation of neutrophils in vitro and in vivo. To examine anti-mBP180 IgG-induced neutrophil activation in vitro, mouse neutrophils from WT and Mac-1 KO mice were stimulated with pathogenic anti-mBP180 IgG (R530) plus mBP180 antigen, and the supernatant was then assayed for MMP-9 (A) and NE (B) by MMP-9 colorimetric and NE enzymatic activity assay, respectively. Mac-1 KO and WT neutrophils showed no difference in neutrophil release of these proteinases. To examine anti-mBP180 IgG-induced neutrophil activation in vivo, WT and Mac-1 KO mice were injected intradermally with pathogenic anti-mBP180 IgG R530, and extracellular levels of released MMP-9 (C) and NE (D) in the IgG injection sites at 4- and 24-hour time points were quantified by MMP-9 colorimetric and NE enzymatic activity assays. Data shown are the mean ± SEM.