Table 2.

Percentage of CXCR-4 expression on CD34⁺ cells

Patient ID	Percent of CXCR-4 on CD34+
Engrafters
9	2.2
29*	4.1
26*	19.1
8	21.5
27	22.2
28	24.5
10	63.3
24*	80.4
5	95.3
20*	96.1
14	98.9
Mean ± SD	48.0 ± 39.0
Nonengrafters
41	4.1
45*	8.4
34*	22.5
35	34.9
42	54.2
47	65
44	69.5
46*	75.9
43	73.4
39*	98.5
Mean ± SD	50.6 ± 9.4

AML cells from 21 different patients were labeled with antibodies to CD34 and CXCR-4. CXCR-4 expression is displayed as a percentage of CD34⁺ cells. There did not seem to be a difference in CXCR-4 expression levels between AML cases capable of NOD/SCID engraftment and those not able to do so. Of note, the highest CXCR-4 expression was observed in a nonengrafter and the lowest was seen in an engrafting AML sample.

^*Samples tested for intracellular calcium release from CD34⁺ cells when stimulated with 100 ng/mL SDF-1 as described in “Calcium flux measurement.” Student paired t test: 1.0