Table 5.

Residues in the hTF N-lobe that may interact with the human TFR compared to residues at equivalent positions in rabbit TF and pig TF.

	N1-subdomain					N2-subdomaina
Bind TFRb		71	72	73	74				142	143	144	145
HUMAN TF		Y	L	A	P				P	R	K	P
RABBIT TF		G	L	T	P				P	R	K	P
PIG TF		G	L	A	P				P	R	K	P
Do not Bind TFR
BOVINE TF		G	L	K	P				P	Q	E	S
CHICKEN oTF		G	L	A	P				I	E	S	G
HUMAN LTF		G	L	A	P				P	P	E	P
Bind TFRc	89	90	91	92	93	94	95	96	97	98	99	100	101	102
HUMAN TF	E	D	P	Q	T	F	Y	Y	A	V	A	V	V	K
RABBIT TF	E	N	P	K	T	F	Y	Y	A	V	A	V	L	K
PIG TF	D	N	P	Q	T	H	Y	Y	A	V	A	V	V	K
Do not Bind TFR
BOVINE TF	D	N	P	Q	T	H	Y	Y	A	V	A	V	V	K
CHICKEN oTF	E	G	S	T	T	S	Y	Y	A	V	A	V	V	K
HUMAN LTF	R	Q	P	R	T	H	Y	Y	A	V	A	V	V	K

^aResidues in the N2- subdomain are highlighted in aqua.

^bResidues 71–74 and 142–145 were identified by the Cryo-EM model (23).

^cResidues 89–102 were identified by radiation foot printing (79).