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. 2007 May 7;104(20):8218–8222. doi: 10.1073/pnas.0700567104

Fig. 4.

Fig. 4.

Nanoinjection of QDot streptavidin conjugates into a target HeLa cell. (A) Fluorescence image of the cells before nanoinjection. (B) Combined bright-field and fluorescence image of the cells before nanoinjection. The inserted arrow indicates the target cell. The dark shape in the lower left corner is the AFM cantilever. (C) Fluorescence image of the cells after the nanoinjection, showing fluorescent QDot streptavidin conjugates released inside the target cell. (D) Combined bright-field and fluorescence image of the cells after the nanoinjection. The QDot streptavidin conjugates are shown in red. The dark shape in the upper left corner is the retracted AFM cantilever. (E) Combined bright-field and fluorescence image of another four examples of HeLa cells after nanoinjection of QDot streptavidin. In all cases, fluorescence images were acquired with λex = 415 nm and data collection with a 655-nm filter. Images are 70 × 70 μm in A–D and 30 × 30 μm in E.